During gel electrohporesis, what would happen?

What effect will be observed in the stained band of DNA in an Agarose gel


a. If the casting tray is moved or jarred while agarose is solidifying (will it distort the gel? tear it, break it?)


b. if the gel is run at a very high voltage (the results would come faster but would they be blurry, or inaccurate)


c. if a large air bubble or clump is allowed to set in agarose?


d. if too much DNA is loaded within a lane

During gel electrohporesis, what would happen?
a. yeah, it just depends on how far along the gel is, you'd be able to tell if there was tear or something otherwise it would be ok.


b. at higher voltages, it would make the bands kind of distorted like you said. i think b/c it would heat up the gel and kind of start to melt it.


c. for the bubble, it would prevent the band(s) from traveling through that part and probably make it distorted, depending on how big it is and how much in the way it was.


d. if you load too much DNA, your bands may not be discernable enough for you see them clearly. especially if you ran large pieces on a gel that wasnt at a low enough percentage.

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